Journal: OncoTargets and therapy

Article Title: Up-regulated LncRNA-ATB regulates the growth and metastasis of cholangiocarcinoma via miR-200c signals

doi: 10.2147/OTT.S217676

Figure Lengend Snippet: Knockdown of Lnc-ATB inhibits CCA cell migration in vitro. The HUCCT1 and RBE cells were transfected with siRNA-Lnc-ATB and miR-200c inhibitor, the migration of ( A and C ) HUCCT1 and ( B and D ) RBE cells were analyzed by Transwell assay. Five random views were selected to count the cells. ** P <0.01, *** P <0.001, data represent the mean ± SD. Abbreviations: CCA, cholangiocarcinoma; NC, normal control.

Article Snippet: The Corning Polycarbonate Membrane Insert transwell chambers (Product #3422; Corning Costar Corp, Cambridge, MA, USA) was used to perform migration assays.

Techniques: Knockdown, Migration, In Vitro, Transfection, Transwell Assay, Control

Journal: Scientific Reports

Article Title: Prognostic factors and Doxorubicin involved in malignant progression of meningioma

doi: 10.1038/s41598-023-28996-0

Figure Lengend Snippet: Doxorubicin inhibits proliferation, migration and invasion of meningioma. ( A ) Correlation of compound with differently expressed mRNA: Heatmap showing compounds (top) from the CMap shares mechanisms of action and the mechanisms of action was sorted by descending number of compounds (right); “ComplexHeatmap” R package (version 3.15) was used to graph the result. ( B ) The molecular structure of Doxorubicin; ( C ) The dose-curve of Doxorubicin in IOMM-Lee cell line; ( D ) CCK-8 assay of the cell proliferation ability in IOMM-Lee cells upon Doxorubicin or DMSO treatment; ( E ) EdU assay of the cell proliferation ability in IOMM-Lee cells upon Doxorubicin or DMSO treatment. Scale bar: 125 μm; ( F ) Statistical analysis of the EdU-positive cell ratio; ( G ) Wound healing assay showed delayed closure of the wound gap upon Doxorubicin treatment. ( H ) Statistical analysis of the cell migration in the scratch wound healing assays. ( I ) Transwell invasion and migration assay of IOMM-Lee cells upon Doxorubicin or DMSO treatment. ( J ) Statistical analysis of the cell numbers passing through the transwell chamber. All data were the means ± SD. Results triplicate and repeated at least three times. Results are expressed as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Migration or invasion assays were performed using 24-well plates inserted by 8-μm pore size transwell filter insert (Costar 3422 [Corning, Corning, New York, USA]) with or without pre-coated diluted Matrigel (1:8) (Becton Dickinson and Co., Franklin Lakes, NJ, USA).

Techniques: Migration, CCK-8 Assay, EdU Assay, Wound Healing Assay

Journal: Frontiers in Genetics

Article Title: Lnc-HSD17B11-1:1 Functions as a Competing Endogenous RNA to Promote Colorectal Cancer Progression by Sponging miR-338-3p to Upregulate MACC1

doi: 10.3389/fgene.2020.00628

Figure Lengend Snippet: lnc-HSD17B11-1:1 promotes cell proliferation, tumor growth, migration and invasion in vitro and in vivo in CRC. (A) Relative expression of lnc-HSD17B11-1:1 in CRC and NCM460 cell lines was examined by qRT-PCR. (B) Relative expression of lnc-HSD17B11-1:1 in HCT116 and SW480 cells transfected with lnc-HSD17B11-1:1 expression vector, mock, sh-lnc or sh-NC, respectively. (C) Growth curves of cells of various groups were evaluated by CCK-8 assays. (D,E) Colony formation assays were detected by proliferation of cells transfected with indicated vectors. (F,G) Cell migration capacities were conducted by wound healing assays after transfection (magnification, ×50). Scale bar, 200μm. (H,I) Cell invasion abilities were indicated by transwell assays in each group (magnification, ×100). Scale bar, 100 μm. (J) Images of subcutaneous xenotransplantation of tumors in each group was photographed ( n = 4). (K) The weight of each group was displayed. Data were showed as mean ± SD, ** P < 0.01, *** P < 0.001, N.S, non-significant.

Article Snippet: Cell invasion assay was carried out using Corning Polycarbonate Membrane insertion transwell chamber (Product #3422, Corning Costar Corp., Cambridge, MA, United States). cells (1 × 10 5 ) in serum-free media were placed into the upper chamber while media containing 20% FBS was placed in the lower chamber as a chemoattractant to perform migration assays (without Matrigel) and invasion assays (with Matrigel, Sigma) at 24 h post-transfection.

Techniques: Migration, In Vitro, In Vivo, Expressing, Quantitative RT-PCR, Transfection, Plasmid Preparation, CCK-8 Assay

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Bringing Safe and Standardized Cell Therapies to Industrialized Processing for Burns and Wounds

doi: 10.3389/fbioe.2020.00581

Figure Lengend Snippet: FE002-SK2 tiered biobanking and GMP tec-transfers. The robust biobanking protocols devised for the FE002-SK2 progenitor fibroblasts allowed for successful technology transfers to BioReliance (Scotland), Transwell Biotech Co. Ltd. (Taiwan), and the CHUV CPC (Epalinges/Lausanne, Switzerland). Varying banking nomenclatures exist between the different groups, the pertinent passage numbers being those used by BioReliance. Quantities of vials listed exclude those consumed for recovery studies and release testing. Pre-clinical safety testing was performed on the EOPCB by BioReliance. Clinical applications comprise the ongoing use of PBBs in the CHUV Burn Center, the pending Priority Project Bru_PBB and both clinical trials sponsored in Asia by TWB.

Article Snippet: Sterile Transwell® inserts (6.5 mm diameter polycarbonate membranes with 8 μm pores, N°3422, Corning®, USA) and 24-well cell culture plates (N°353047, Corning®, USA) served as the experimental scaffold.

Techniques: Clinical Proteomics

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Bringing Safe and Standardized Cell Therapies to Industrialized Processing for Burns and Wounds

doi: 10.3389/fbioe.2020.00581

Figure Lengend Snippet: Representative imaging of migrated primary human keratinocytes (DAPI staining) in Transwell® migration assay (left and middle columns). Representative imaging includes middle fields of 3 wells/group. Contamination of Transwells® by FE002-SK2 progenitor fibroblasts was negligible (right column). Mean cell counts are listed in .

Article Snippet: Sterile Transwell® inserts (6.5 mm diameter polycarbonate membranes with 8 μm pores, N°3422, Corning®, USA) and 24-well cell culture plates (N°353047, Corning®, USA) served as the experimental scaffold.

Techniques: Imaging, Staining, Transwell Migration Assay

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Bringing Safe and Standardized Cell Therapies to Industrialized Processing for Burns and Wounds

doi: 10.3389/fbioe.2020.00581

Figure Lengend Snippet: Average counts of migrated primary keratinocytes in Transwell® migration assay, in the absence and presence of FE002-SK2 progenitor fibroblasts in the culture wells.

Article Snippet: Sterile Transwell® inserts (6.5 mm diameter polycarbonate membranes with 8 μm pores, N°3422, Corning®, USA) and 24-well cell culture plates (N°353047, Corning®, USA) served as the experimental scaffold.

Techniques: Transwell Migration Assay